Characterization of the distributions of collagen and PGs content in the decellularized book-shaped enthesis scaffolds by SR-FTIR was written by Shi, Qiang;Chen, Can;Li, Muzhi;Chen, Yang;Xu, Yan;Hu, Jianzhong;Liu, Jun;Lu, Hongbin. And the article was included in BMC Musculoskeletal Disorders in 2021.Application of 1461-15-0 This article mentions the following:
Bone-tendon interface (enthesis) plays a pivotal role in relaxing load transfer between otherwise structurally and functionally distinct tissue types. Currently, decellularized extracellular matrix (DEM) from enthesis provide a natural three-dimensional scaffold with tissue-specific orientations of extracellular matrix mols. for enthesis regeneration, however, the distributions of collagen and PGs content in the decellularized book-shaped enthesis scaffolds from rabbit rotator cuff by SR-FTIR have not been reported. Native enthesis tissues (NET) harvested from rabbit rotator cuff were sectioned into cuboid (about 30 mm × 1.2 mm × 10 mm) for decalcification. The decellularized book-shaped enthesis scaffolds and intrinsic ultrastructure were evaluated by histol. staining and SEM (SEM), resp. The distributions of collagen and PGs content in the decellularized book-shaped enthesis scaffolds from rabbit rotator cuff were also measured innovatively by SR-FTIR. The decellularized book-shaped enthesis scaffolds from rabbit rotator cuff were successfully obtained. Histomorphol. and SEM evaluated the effect of decellularization and the structure of extracellular matrix during decellularization. After mech. testing, the failure load in the NET group showed significantly higher than that in the DEM group (P < 0.05). Meanwhile, the stiffness of the DEM group was significantly lower than the NET group. Furthermore, the distributions of collagen and PGs content in the decellularized book-shaped enthesis scaffolds were decreased obviously after decellularization by SR-FTIR quant. anal. SR-FTIR was applied innovatively to characterize the histol. morphol. of native enthesis tissues from rabbit rotator cuff. Moreover, this technol. can be applied for quant. mapping of the distribution of collagen and PGs content in the decellularized book-shaped enthesis scaffolds. In the experiment, the researchers used many compounds, for example, 2,2′,2”,2”’-(((3′,6′-Dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9′-xanthene]-2′,7′-diyl)bis(methylene))bis(azanetriyl))tetraacetic acid (cas: 1461-15-0Application of 1461-15-0).
2,2′,2”,2”’-(((3′,6′-Dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9′-xanthene]-2′,7′-diyl)bis(methylene))bis(azanetriyl))tetraacetic acid (cas: 1461-15-0) belongs to benzofurans derivatives. Benzofuran derivatives are one of the most important oxygen-containing heterocycles. Benzofurans are stable towards alkali and readily polymerize on treatment with sulfuric acid, due to which they are useful for the preparation of low cost chemically relatively inert resins.Application of 1461-15-0
Referemce:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem