Quality Control of Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate. The reaction of aromatic heterocyclic molecules with protons is called protonation. Aromatic heterocycles are more basic than benzene due to the participation of heteroatoms. Compound: Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate, is researched, Molecular C18H20N2O12, CAS is 70539-42-3, about A new cleavable reagent for cross-linking and reversible immobilization of proteins. Author is Abdella, Peter M.; Smith, Paul K.; Royer, Garfield P..
A new bifunctional reagent was prepared for the crosslinking and reversible immobilization of proteins through their amino groups. This compound, ethylene glycol bis(succinimidyl succinate) (I), reacted rapidly with proteins, at pH 7 and at high dilution The resulting protein crosslinks were readily cleaved at pH 8.5 using hydroxylamine for 3-6 h at 37°. Substantial enzymic activity was observed with lactate dehydrogenase after such reversible crosslinking. Trypsin immobilized on agarose using this reagent retained full sp. activity, was stable for weeks in the cold, and could be released with hydroxylamine at 25°. This compound appears suitable for studies involving proteins with essential disulfide linkages.
After consulting a lot of data, we found that this compound(70539-42-3)Quality Control of Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate can be used in many types of reactions. And in most cases, this compound has more advantages.
Reference:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem