The reaction of an aromatic heterocycle with a proton is called a protonation. One of articles about this theory is 《Thermostabilized chemical derivatives of horseradish peroxidase》. Authors are Ryan, Orlaith; Smyth, Malcolm R.; Fagain, Ciaran O..The article about the compound:Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinatecas:70539-42-3,SMILESS:O=C(ON1C(CCC1=O)=O)CCC(OCCOC(CCC(ON2C(CCC2=O)=O)=O)=O)=O).Electric Literature of C18H20N2O12. Through the article, more information about this compound (cas:70539-42-3) is conveyed.
Horseradish peroxidase finds a variety of uses in anal., immunol., organic synthesis, and biosensors. Although moderately stable, its applicability to biosensors and other fields would be greatly enhanced if it could be made yet more stable. Appropriate chem. modification can substantially stabilize enzymes. Here the authors describe the use of bis-imidates and of bis-succinimides to modify free amino groups of com. horseradish peroxidase under mild conditions of pH and temperature Imidates yielded a marginal stabilization. Some of the succinimide derivatives, however, are much more thermostable than the native enzyme. Apparent half-lives indicate stabilizations of 6- to 23-fold, depending on the bis-succinimide used. These modifications preserve the carbohydrate side chains for subsequent reaction or immobilization.
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Reference:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem