Simultaneous screening of homotaurine and taurine in marine macro-algae using liquid chromatography-fluorescence detection was written by Mehdinia, Ali;Rostami, Simindokht;Dadkhah, Sahar;Fumani, Neda Sheijooni. And the article was included in Journal of the Iranian Chemical Society in 2017.Reference of 38183-12-9 This article mentions the following:
Simultaneous anal. of homotaurine and its homologous, taurine, is a highly challenging issue, especially in matrixes they exist simultaneously. A simple precolumn derivatization procedure combined with high-performance liquid chromatog.-fluorescence detection was developed for simultaneous determination of homotaurine and taurine in marine macro-algae. The analytes were derivated with o-phthalaldehyde at an ambient temperature and alk. medium. Calibration curves were linear in the ranges of 50-2500μg L-1 for homotaurine and 100-2500μg L-1 for taurine with the coefficients of determination higher than 0.998. Limits of detection of homotaurine and taurine were 15 and 30μg L-1, resp. Intraday (n = 6) and inter-day (n = 4) precisions of the method were satisfactory with relative standard deviations less than 6.0%. Good recoveries (>94%) were acquired by the method for extraction of homotaurine and taurine from algae matrixes. Liquid chromatog.-mass spectrometry was also used to confirm detection of the analytes in algae samples. The data suggest that the method was successfully applied to simultaneous determination of homotaurine and taurine in algae samples. In the experiment, the researchers used many compounds, for example, 4-Phenyl-3H,3’H-spiro[furan-2,1′-isobenzofuran]-3,3′-dione (cas: 38183-12-9Reference of 38183-12-9).
4-Phenyl-3H,3’H-spiro[furan-2,1′-isobenzofuran]-3,3′-dione (cas: 38183-12-9) belongs to benzofurans derivatives. Benzofuran is the “”parent”” of many related compounds with more complex structures. For example, psoralen is a benzofuran derivative that occurs in several plants. Introduction of benzofurans in organic synthesis, particularly drug synthesis, involves generally the use of their metalated species as nucleophiles in addition reactions or in metal-catalysed cross-coupling reactions.Reference of 38183-12-9
Referemce:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem