Using 2′,7′-Dichlorofluorescein (DCF) or 2′,7′-dichlorodihydrofluorescein diacetate (DCFH2-DA) to measure reactive oxygen species in erythrocytes was written by Hans, Chander;Saini, Rahul;Sachdeva, Man Updesh Singh;Sharma, Prashant. And the article was included in Biomedicine & Pharmacotherapy in 2021.Recommanded Product: 76-54-0 This article mentions the following:
A polemic in response to Hao et. al., Biomed. Pharmacother. 122 (2020), 109677 is reported. Hao et. al., stated that acetylshikonin induces apoptosis of human leukemia cell line K562 by inducing S phase cell cycle arrest, modulating ROS accumulation, depleting Bcr-Abl and blocking NF-&kappaB signaling. The comprehensive study by Hao and colleagues is on the effect of acetylshikonin on the K562 cell line including modulation of intracellular reactive oxygen species (ROS) accumulation. However, the authors like to point out an error in reagent nomenclature/citation in their paper. This is actually a recurring issue in literature and has the potential to affect other research results just as it undermined theirs, recently. For a recent pilot experiment on human erythrocytes, the authors needed to measured intracellular ROS generation in various hematol. disorders. Guided by several prior publications, including Hao et al., a 2′,7′-dichlorodihydrofluorescein diacetate (DCFH2-DA)-based flow cytometric experiment was designed. This is potentially confusing for future buyers who may land there following the SKU number wrongly quoted in the publications. In the experiment, the researchers used many compounds, for example, 2′,7′-Dichloro-3′,6′-dihydroxy-3H-spiro[isobenzofuran-1,9′-xanthen]-3-one (cas: 76-54-0Recommanded Product: 76-54-0).
2′,7′-Dichloro-3′,6′-dihydroxy-3H-spiro[isobenzofuran-1,9′-xanthen]-3-one (cas: 76-54-0) belongs to benzofurans derivatives. Benzofurans are compounds with a planar structure having 10 pi electrons that include the lone pair on oxygen atom, which makes it more susceptible to electrophilic attack. In nature, benzofurans have occupied an important role among the plant phenols & several pharmacologically active compounds.Recommanded Product: 76-54-0
Referemce:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem