So far, in addition to halogen atoms, other non-metallic atoms can become part of the aromatic heterocycle, and the target ring system is still aromatic.Shi, An-Guo; Deth, Richard C. researched the compound: Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate( cas:70539-42-3 ).Formula: C18H20N2O12.They published the article 《Precoupling of alpha-2B adrenergic receptors and G-proteins in transfected PC-12 cell membranes: influence of pertussis toxin and a lysine-directed cross-linker》 about this compound( cas:70539-42-3 ) in Journal of Pharmacology and Experimental Therapeutics. Keywords: alpha2B adrenergic receptor precoupling G protein. We’ll tell you more about this compound (cas:70539-42-3).
The ability of pertussis toxin (PTX) pretreatment to alter the binding of [3H]rauwolscine (RAU) to alpha-2B adrenergic receptors expressed in PC12 cells was examined PTX caused a 30% increase in the Bmax for [3H]RAU and reduced its KD, whereas in the added presence of Na+ and Gpp(NH)p binding was increased to 75% above the level in untreated membranes. Because all three agents act to reduce receptor/G-protein affinity, the increased binding may reflect extensive precoupling of the alpha-2B receptor. The affinity of the agonist epinephrine in displacing [3H]RAU was normally reduced by both Na+ and Gpp(NH)p; however, in PTX-treated membranes the effect of Gpp(NH)p was eliminated, and Na+ remained effective. The lysine-directed crosslinking reagent ethyleneglycol bis(succinimidyl)succinate (EGS) was utilized to cross-link precoupled receptor and G-protein. Maximal [3H]RAU binding was reduced by EGS in a time- and dose-dependent manner, and this action was reversed by prior incubation with Na+ and Gpp(NH)p, suggesting that EGS did indeed cross-link receptor and G-protein. RAU and epinephrine each provided protection against the effect of EGS. The inclusion of Na+ and Gpp(NH)p during [3H]RAU binding studies was able to restore maximal binding in EGS-treated membranes to the same level as untreated membranes. These results indicate that in the absence of Na+ and Gpp(NH)p at least 40% of the total alpha-2B adrenergic receptors in these membranes exist as a precoupled receptor/G-protein complex which fails to bind [3H]RAU.
Although many compounds look similar to this compound(70539-42-3)Formula: C18H20N2O12, numerous studies have shown that this compound(SMILES:O=C(ON1C(CCC1=O)=O)CCC(OCCOC(CCC(ON2C(CCC2=O)=O)=O)=O)=O), has unique advantages. If you want to know more about similar compounds, you can read my other articles.
Reference:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem