Quality Control of Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate. Aromatic compounds can be divided into two categories: single heterocycles and fused heterocycles. Compound: Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate, is researched, Molecular C18H20N2O12, CAS is 70539-42-3, about Conformational Adaptation of Apolipoprotein A-I to Discretely Sized Phospholipid Complexes. Author is Bhat, Shaila; Sorci-Thomas, Mary G.; Tuladhar, Rubina; Samuel, Michael P.; Thomas, Michael J..
The conformational constraints for apoA-I bound to recombinant phospholipid complexes (rHDL) were attained from a combination of chem. crosslinking and mass spectrometry. Mol. distances were then used to refine models of lipid-bound apoA-I on both 80 and 96 Å diameter rHDL particles. To obtain mol. constraints on the protein bound to phospholipid complexes, three different lysine-selective homo-bifunctional cross-linkers with increasing spacer arm lengths (i.e., 7.7, 12.0, and 16.1 Å) were reacted with purified, homogeneous recombinant 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) apoA-I rHDL complexes of each diameter Cross-linked dimeric apoA-I products were separated from monomeric apoprotein using 12% SDS-PAGE, then subjected to in-gel trypsin digest, and identified by MS/MS sequencing. These studies aid in the refinement of our previously published mol. model of two apoA-I mols. bound to ∼150 mols. of POPC and suggest that the protein hydrophobic interactions at the N- and C-terminal domains decrease as the number of phospholipid mols. or “”lipidation state”” of apoA-I increases. Thus, it appears that these incremental changes in the interaction between the N- and C-terminal ends of apoA-I stabilize its tertiary conformation in the lipid-free state as well as allowing it to unfold and sequester discrete amounts of phospholipid mols.
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Reference:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem