Recommanded Product: 70539-42-3. The protonation of heteroatoms in aromatic heterocycles can be divided into two categories: lone pairs of electrons are in the aromatic ring conjugated system; and lone pairs of electrons do not participate. Compound: Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate, is researched, Molecular C18H20N2O12, CAS is 70539-42-3, about Analysis of the molecular organization of recombinant human tumor necrosis factor (rTNF) in solution using ethylene glycolbis(succinimidylsuccinate) as the cross-linking reagent. Author is Lam, Kit S.; Scuderi, Philip; Salmon, Sydney E..
Anal. of chem. cross-linked recombinant human tumor necrosis factor (rTNF) on SDS-PAGE revealed the presence of 3 distinct mol. forms which correspond to trimers, dimers, and monomers. The crosslinking procedure appeared to enhance the antigenicity of these mol. forms of rTNF as determined by Western blot anal. Crosslinking was unaffected by 2% Nonidet P-40, high concentrations of human serum albumin, or 60% normal human serum. SDS (0.08%) completely abolished the crosslinking of rTNF. The rTNF, at a concentration as low as 0.8 ng/mL, exists solely in the trimeric form in solution under nondenaturing conditions.
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Reference:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem