Reference of Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate. The mechanism of aromatic electrophilic substitution of aromatic heterocycles is consistent with that of benzene. Compound: Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate, is researched, Molecular C18H20N2O12, CAS is 70539-42-3, about Characterization of a bombesin receptor on Swiss mouse 3T3 cells by affinity cross-linking. Author is Sinnett-Smith, James; Zachary, Ian; Rozengurt, Enrique.
A cell surface protein in Swiss 3T3 cells of apparent Mr 75,000-85,000 was identified previously by chem. crosslinking as a major component of the receptor for peptides of the bombesin family in these cells. Because bombesin-like peptides may interact with other cell surface mols., it was important to establish the correlation between receptor binding and functions of this complex and further characterize the Mr 75,000-85,000 cross-linked protein. Detailed time courses carried out at different temperatures demonstrated that the Mr 75,000-85,000 affinity-labeled band was the earliest cross-linked complex detected in Swiss 3T3 cells incubated with 125I-labeled gastrin-releasing peptide (125I-GRP). Furthermore, the ability of various nonradioactive bombesin agonists and antagonists to block the formation of the Mr 75,000-85,000 cross-linked complex correlated extremely well (r = 0.994) with the relative capacity of these peptides to inhibit 125I-GRP specific binding. Pretreatment with unlabeled GRP for up to 6 h caused only a slight decrease in both specific 125I-GRP binding and the affinity labeling of the Mr 75,000-85,000 protein. The cross-linked complex is a glycoprotein. First, solubilized affinity labeled Mr 75,000-85,000 complex applied to wheat germ lectin-Sepharose columns was eluted by addition of 0.3M N-acetyl-D-glucosamine. Second, treatment with endo-β-N-acetylglucosaminidase F reduced the apparent mol. weight of the affinity-labeled band from 75,000-85,000 to 43,000, indicating the presence of N-linked oligosaccharide groups.
After consulting a lot of data, we found that this compound(70539-42-3)Reference of Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate can be used in many types of reactions. And in most cases, this compound has more advantages.
Reference:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem