The chemical properties of alicyclic heterocycles are similar to those of the corresponding chain compounds. Compound: Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate, is researched, Molecular C18H20N2O12, CAS is 70539-42-3, about Early cleavage of ethylene glycol bis(succinimidylsuccinate) (EGS)-linker moieties during enzymatic digestion of cross-linked proteins, the main research direction is sulfo EGS peptide enzymic digestion crosslinked protein.HPLC of Formula: 70539-42-3.
It has become popular to combine the crosslinking of proteins with mass spectrometry (MS) to study their tertiary structure or interactions. There are several difficulties with the method which make it a challenge. They include the limited availability of crosslinked peptides for MS anal. due to low yield of the crosslinking reaction and unwanted side chem. Recently, the authors have pointed out the uncontrolled quenching of azide linkers by buffer components rendering them almost useless for the intended task. Here, the authors describe a phenomenon involving cleavable crosslinkers. They have been designed to overcome the drawback that crosslinked peptides provide poor gas-phase fragmentation mass spectra because of their branched structure. Moreover, isotope coding on such linkers may assist in finding the low-abundance crosslinked peptides in mass spectra as described for ethylene glycol bis(succinimidylsuccinate) (EGS). In some of the authors’ experiments, this compound provided better crosslinking yields compared to other N-hydroxysuccinimide-derived reagents as detected by one-dimensional sodium dodecyl sulfate PAGE. For best results from crosslinking experiments using cleavable agents such as EGS, careful control of sample preparation protocols for early hydrolysis products is advisable.
After consulting a lot of data, we found that this compound(70539-42-3)HPLC of Formula: 70539-42-3 can be used in many types of reactions. And in most cases, this compound has more advantages.
Reference:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem