The preparation of ester heterocycles mostly uses heteroatoms as nucleophilic sites, which are achieved by intramolecular substitution or addition reactions. Compound: Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate( cas:70539-42-3 ) is researched.Application of 70539-42-3.Massague, Joan; Guillette, Barbara J.; Czech, Michael P. published the article 《Affinity labeling of multiplication stimulating activity receptors in membranes from rat and human tissues》 about this compound( cas:70539-42-3 ) in Journal of Biological Chemistry. Keywords: multiplication stimulating activity receptor; membrane multiplication stimulating activity receptor; adipose multiplication stimulating activity receptor; placenta multiplication stimulating activity receptor; liver multiplication stimulating activity receptor. Let’s learn more about this compound (cas:70539-42-3).
Plasma membranes from rat adipocytes and liver and from human placenta were labeled by covalent crosslinking to membrane-bound 125I-labeled multiplication-stimulating activity (125I-MSA) with 3 different bishydroxysuccinimide esters: disuccinimidyl suberate, disuccinimidyl succinate, and ethyleneglycolylbis(succinimidyl succinate). Dodecyl sulfate-polyacrylamide gel electrophoresis and autoradiog. anal. of the 125I-MSA-labeled material in the presence of dithiothreitol reveals 1 single-labeled protein migrating with an apparent Mr = 255,000 regardless of the kind and concentration of crosslinker used. Electrophoresis in the absence of reductant indicates that the affinity-labeled species is not disulfide-linked to any other protein in the native plasma membrane, but contains internal disulfide bonds that compact its structure. The labeling of the Mr = 255,000 species increases with increasing concentrations of 125I-MSA of 0.3-3 nM. Labeling is abolished in a competitive manner by nonradioactive MSA but not by similar concentrations of insulin, proinsulin, or epidermal growth factor in all 3 tissues examined The unique labeling of this Mr = 225,000 membrane component and its selective inhibition by MSA suggest that this protein is a plasma membrane receptor for MSA.
Different reactions of this compound(Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate)Application of 70539-42-3 require different conditions, so the reaction conditions are very important.
Reference:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem