Rapid analysis of sulfamethoxazole residue in milk based on fluorescence spectroscopy coupled with chemometrics was written by Zhang, Yan-ping;Feng, Shi-yun;Zhou, Peng;Liu, Xiao-ming. And the article was included in Shipin Gongye Keji in 2013.HPLC of Formula: 38183-12-9 This article mentions the following:
Antibiotic residues in animal tissues and products could bring risks to the health of consumers. The commonly employed methods for detecting antibiotic residues often involved time-consuming protocols, whereas the fluorescence method was characterized as rapid and sensitive. In this study, a method was developed for rapid screening of sulfamethoxazole residues in milk with fluorescence spectroscopy and the chemometrics tools. The established model was applied to 6 brands of com. milk including pasteurized and ultra heat-treated (UHT) milk (whole, skimmed, semi-skimmed, high calcium). The results showed a significant discrimination by partial least squares discriminant anal. (PLS-DA) between the neg. and pos. milk samples with a 3.12% of false neg. probability, which was below 5% required by the European Union. And quantification could be acquired by further anal. with partial least squares (PLS) with a recovery between 87.9%-131.6%. The correlation coefficient was 0.9992 in the linear range of 0-210 μg/kg, and the detection capability (CCβ) and detection limit (CCα) were 2.54 μg/L and 5.07 μg/L, resp. The average relative standard error of the prediction model was 3.24%. This method showed potential in rapid screening and anal. of sulfamethoxazole residues in milk. In the experiment, the researchers used many compounds, for example, 4-Phenyl-3H,3’H-spiro[furan-2,1′-isobenzofuran]-3,3′-dione (cas: 38183-12-9HPLC of Formula: 38183-12-9).
4-Phenyl-3H,3’H-spiro[furan-2,1′-isobenzofuran]-3,3′-dione (cas: 38183-12-9) belongs to benzofurans derivatives.Benzofuran is one of the most significant oxygen-containing heterocycles consisting of fused benzene and furan ring, which are widely presented in various naturally occurring and synthetically active compounds. Benzofurans have also made significant and distinctive contributions to biology. They exhibit several biological activities that range from antioxidant, antitubercular, antiplasmodial, insecticidal.HPLC of Formula: 38183-12-9
Referemce:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem