Epoxy compounds usually have stronger nucleophilic ability, because the alkyl group on the oxygen atom makes the bond angle smaller, which makes the lone pair of electrons react more dissimilarly with the electron-deficient system. Compound: (R)-Ethyl 4-chloro-3-hydroxybutanoate, is researched, Molecular C6H11ClO3, CAS is 90866-33-4, about Purification and characterization of a novel carbonyl reductase with high stereo-selectivity.Recommanded Product: 90866-33-4.
A novel NADPH dependent carbonyl reductase was separated from Candida parapsilosis CCTCC 203011. The enzyme gave a single band on SDS-PAGE, which was purified through ammonium sulfate, DEAE Sepharose FF, Phenyl-Sepharose FF and Blue Sepharose FF chromatog. from cell-free extract The mol. mass of the enzyme was about 30 kD. The optimum pH and temperature for reduction were 4.5 and 35°C, resp. The Cu2+ had strong restrictive effect on enzyme activity. In addition, the carbonyl reductase was an enzyme with high substrate specificity and stereo-selectivity, and showed high asym. reduction activity toward α-hydroxyacetophenone and Et 4-chloroacetoacetate. For the asym. reduction of α-hydroxyacetophenone and Et 4-chloroacetoacetate, (S)-1-phenyl-1,2-ethanediol and (R)-Et 4-chloro-3-hydroxybutanoate were produced by the purified enzyme, with the 100% and 94.3% e.e value, resp. So the enzyme could be one of the effective biocatalysts for asym. synthesis of chiral alcs. The amino acid sequences of one peptide from the purified enzyme were analyzed by LC-MASS-MASS, and the carbonyl reductase showed some identity to the hypothetical protein CaO19.10414 reported.
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Reference:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem