Li, Jing et al. published their research in Yaowu Fenxi Zazhi in 2014 | CAS: 38183-12-9

4-Phenyl-3H,3’H-spiro[furan-2,1′-isobenzofuran]-3,3′-dione (cas: 38183-12-9) belongs to benzofurans derivatives. Benzofuran is the “”parent”” of many related compounds with more complex structures. For example, psoralen is a benzofuran derivative that occurs in several plants. Substituted benzofurans find applications such as fluorescent sensors, oxidants, in drug discovery, and in another field of chemistry and agriculture.Safety of 4-Phenyl-3H,3’H-spiro[furan-2,1′-isobenzofuran]-3,3′-dione

A fluorometric assay to determine average modification rate of three kinds of PEG-rhGHs was written by Li, Jing;He, Hui;Cheng, Su-yuan;Liang, Cheng-gang. And the article was included in Yaowu Fenxi Zazhi in 2014.Safety of 4-Phenyl-3H,3’H-spiro[furan-2,1′-isobenzofuran]-3,3′-dione This article mentions the following:

The average rate of 3 different PEG modifications of rhGH by deriving free amines with fluorescamine was determined MALDI-TOF mass spectrometry was used to detect relative mol. mass of PEG, rhGH and the conjugations to calculate the theor. rate of PEG modification. A fluorometric method was established by optimization and validation through selection of the excitation and emission wavelength, an orthogonal experiment, an interference test of free PEG and the stability study of the reaction. The theor. value of modification rate was 10%, which was validated by MALDI-TOF mass. Validation results were as below: The excitation and emission wavelengths were 390 nm and 480 nm, resp. The pH value of solution had the greatest effect on the result. PEG with low content had no effect on the result. The relative fluorescence unit was stable within the first 8 min. The actual modification rates of three different PEG-rhGHs were 8.8%, 9.8% and 9.5%, resp., which were close to the theor. value. The fluorometric derivatization method is suitable for determination of the average rate of modification for PEG-rhGH. The average modification rate of 3 kinds of PEG-rhGHs is 10%. In the experiment, the researchers used many compounds, for example, 4-Phenyl-3H,3’H-spiro[furan-2,1′-isobenzofuran]-3,3′-dione (cas: 38183-12-9Safety of 4-Phenyl-3H,3’H-spiro[furan-2,1′-isobenzofuran]-3,3′-dione).

4-Phenyl-3H,3’H-spiro[furan-2,1′-isobenzofuran]-3,3′-dione (cas: 38183-12-9) belongs to benzofurans derivatives. Benzofuran is the “”parent”” of many related compounds with more complex structures. For example, psoralen is a benzofuran derivative that occurs in several plants. Substituted benzofurans find applications such as fluorescent sensors, oxidants, in drug discovery, and in another field of chemistry and agriculture.Safety of 4-Phenyl-3H,3’H-spiro[furan-2,1′-isobenzofuran]-3,3′-dione

Referemce:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem