In vitro tetraploidization for the augmentation of wedelolactone in Sphagneticola calendulacea (L.) Pruski was written by Kundu, Suprabuddha;Salma, Umme;Ali, Nasim Md.;Mandal, Nirmal. And the article was included in Acta Physiologiae Plantarum in 2018.Related Products of 524-12-9 The following contents are mentioned in the article:
A practical and reliable method for in vitro tetraploidization of Sphagneticola calendulacea (L.) Pruski [synonym Wedelia chinensis (Osbeck) Merrill] has been established to enhance the production of wedelolactone. Shoot tip and nodal explants from in vitro-grown culture (2n = 50) were exposed to the antimitotic chem., i.e., colchicine, at various concentrations (0, 0.025, 0.05, 0.1, 0.3, and 0.5%; w/v) for 12, 24, 36, 48, and 60 h. The treated explants were then incubated and proliferated on Murashige and Skoog (MS) medium fortified with 0.2 mg l-1 thidiazuron and 0.05 mg l-1 naphthalene acetic acid, followed by root induction in 1.0 mg l-1 indole-3 acetic acid enriched 1/2MS medium. Treatment of shoot tips with 0.05% colchicine for 24 h supported the maximum rate of survival (63.33%) of explants as well as tetraploid induction (42.93%). Morphol., stomatal, and cytol. characteristics along with the secondary metabolite content of the in vitro tetraploids were compared to that of diploids. The recovered tetraploid plants possessed superior plant height, stem diameter, leaf size, root number, and increased length and width of stomata but decreased stomatal frequency. The tetraploid plants demonstrated twice the chromosome number (2n = 4x = 100) than the diploids as confirmed through cytol., spectrophotometry and flow cytometry. High-performance thin-layer chromatog. showed a significant enhancement in the wedelolactone content of tetraploid plants (541.48μg g-1 of dried sample) in comparison to diploid plants (325.43μg g-1 of dried sample), signifying the prospective of this technique for the trade value improvement. This study involved multiple reactions and reactants, such as 1,8,9-Trihydroxy-3-methoxy-6H-benzofuro[3,2-c]chromen-6-one (cas: 524-12-9Related Products of 524-12-9).
1,8,9-Trihydroxy-3-methoxy-6H-benzofuro[3,2-c]chromen-6-one (cas: 524-12-9) belongs to benzofurans derivatives. Benzofuran is the “”parent”” of many related compounds with more complex structures. For example, psoralen is a benzofuran derivative that occurs in several plants. They are also prone to polymerisation in the presence of concentrated mineral acids and Lewis acids.Related Products of 524-12-9
Referemce:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem