Ercin, Ugur; Aribas, Yavuz Kemal; Tefon Aribas, Atike Burcin; Bilgihan, Ayse; Bilgihan, Kamil published their research in Eye (London, United Kingdom) in 2021. The article was titled 《Singlet oxygen formation during accelerated and hyperaccelerated corneal crosslinking: in vitro study》.HPLC of Formula: 5471-63-6 The article contains the following contents:
To evaluate the singlet oxygen (1O2) production of oxygen assisted %0.1 riboflavin and UV-A (UVA) crosslinking therapy (with and without oxygen assistance), in combination with standard, accelerated and hyper-accelerated procedures via an important quantitive marker of 1O2 which is the photo-oxidation of 1,3 diphenylisobenzofuran (DPBF). %0.1 Riboflavin-containing wells were irradiated with UVA light (365-nm wavelength) with or without 2-4-6-8 L/min oxygen flow assistance. Measurements of decrease in absorbance of DPBF were made in 30 mW (hyper-accelerated), 9 mW (accelerated), and 3 mW UV-A (standard) applications, and with addnl. 2-4-6-8 L/min oxygen flow in 30 mW and 2 L/min oxygen flow in 9 mW. A total of 8 different UV-A irradiance with and without oxygen supplementation groups were formed. 2 L/min Oxygen assisted accelerated UV-A irradiance group has shown a greater decrease in DPBF absorbance compared to Dresden protocol. (p = 0.014) Also, Dresden protocol has shown a greater decrease in DPBF compared to all groups except accelerated crosslinking with 2 L/min oxygen. (p < 0.001) Oxygen assisted hyper-accelerated crosslinking groups were showed greater reduction in DPBF absorbance compared to standard crosslinking without oxygen groups. (p < 0.001). Oxygen supplementation may increase the singlet oxygen generation to the similar levels of Dresden Protocol's in accelerated group. Also, more singlet oxygen generation with oxygen supplementation compared to standard UV-A application might be considered to be promising in terms of shortening the crosslinking therapy. In the experimental materials used by the author, we found 1,3-Diphenylisobenzofuran(cas: 5471-63-6HPLC of Formula: 5471-63-6)
1,3-Diphenylisobenzofuran(cas: 5471-63-6) can be used as a fluorescent probe for detection of superoxide anion radical (O2−) inside the membrane lipid layer by DPBF fluorescence quenching method. 1,3-Diphenylisobenzofuran(DPBF) can be used to study the single crystal molecular structure and solution photophysical properties of DPBF.HPLC of Formula: 5471-63-6
Referemce:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem