Silica nanoparticles with thermally activated delayed fluorescence for live cell imaging was written by Crucho, Carina I. C.;Avo, Joao;Nobuyasu, Roberto;N. Pinto, Sandra;Fernandes, Fabio;Lima, Joao C.;Berberan-Santos, Mario N.;Dias, Fernando B.. And the article was included in Materials Science & Engineering, C: Materials for Biological Applications in 2020.Category: benzofurans This article mentions the following:
Thermally activated delayed fluorescence (TADF) has revolutionized the field of organic light emitting diodes owing to the possibility of harvesting non-emissive triplet states and converting them in emissive singlet states. Despite this strong potential, only recently TADF has emerged as a powerful tool to develop metal-free long-lived luminescent probes for imaging and sensing. The application of TADF mols. in aqueous and/or biol. media requires specific structural features that allow complexation with biomols. or enable emission in the aggregated state, in order to retain the delayed fluorescence that is characteristic of these compounds Herein we demonstrate a facile method that maintains the optical properties of solvated dyes by dispersing TADF mols. in nanoparticles. TADF dye-doped silica nanoparticles are prepared using a modified fluorescein fluorophore. However, the strategy can be used with many other TADF dyes. The covalent grafting of the TADF emitter into the inorganic matrix effectively preserves and transfers the optical properties of the free dye into the luminescent nanomaterials. Importantly, the silica matrix is efficient in shielding the dye from solvent polarity effects and increases delayed fluorescence lifetime. The prepared nanoparticles are effectively internalized by human cells, even at low incubation concentrations, localizing primarily in the cytosol, enabling fluorescence microscopy imaging at low dye concentrations In the experiment, the researchers used many compounds, for example, 2′,7′-Dichloro-3′,6′-dihydroxy-3H-spiro[isobenzofuran-1,9′-xanthen]-3-one (cas: 76-54-0Category: benzofurans).
2′,7′-Dichloro-3′,6′-dihydroxy-3H-spiro[isobenzofuran-1,9′-xanthen]-3-one (cas: 76-54-0) belongs to benzofurans derivatives. Benzofuran is a core structural unit found in many naturally occurring compounds with multidirectional biological activities. Benzofurans have also made significant and distinctive contributions to biology. They exhibit several biological activities that range from antioxidant, antitubercular, antiplasmodial, insecticidal.Category: benzofurans
Referemce:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem