Optimization of analytical assay performance of antibody-gated indicator-releasing mesoporous silica particles was written by Costa, Elena;Climent, Estela;Gawlitza, Kornelia;Wan, Wei;Weller, Michael G.;Rurack, Knut. And the article was included in Journal of Materials Chemistry B: Materials for Biology and Medicine in 2020.COA of Formula: C20H10Cl2O5 This article mentions the following:
Antibody-gated indicator delivery (gAID) systems based on mesoporous silica nano- and microparticle scaffolds are a promising class of materials for the sensitive chem. detection of small-mol. analytes in simple test formats such as lateral flow assays (LFAs) or microfluidic chips. Their architecture is reminiscent of drug delivery systems, only that reporter mols. instead of drugs are stored in the voids of a porous host particle. In addition, the pores are closed with macromol. “caps” through a tailored “gatekeeping” recognition chem. so that the caps are opened when an analyte has reacted with a “gatekeeper”. The subsequent uncapping leads to a release of a large number of indicator mols., endowing the system with signal amplification features. Particular benefits of such systems are their modularity and adaptability. With the example of the immunochem. detection of type-I pyrethroids by fluorescent dye-releasing gAID systems, the influence of several tuning modes on the optimization of such hybrid sensory materials is introduced here. In particular, different mesoporous silica supports (from nano- and microparticles to platelets and short fibers), different functionalization routes and different loading sequences were assessed. The materials′ performances were evaluated by studying their temporal response behavior and detection sensitivity, including the tightness of pore closure (through the amount of blank release in the absence of analyte) and the release kinetics. The authors′ results indicate that the better the paratope-accommodating Fab region of the antibody “cap” fits into the host material′s pore opening, the better the closing/opening mechanism can be controlled. Because such materials are well-suited for LFAs, performance assessment included a test-strip format besides conventional assays in suspension. In combination with dyes as indicators and smartphones for read-out, simple anal. tests for use by untrained personnel directly at a point-of-need such as an aeroplane cabin can be devised, allowing for sensitivities down to the μg kg-1 range in <5 min with case-required selectivities. In the experiment, the researchers used many compounds, for example, 2′,7′-Dichloro-3′,6′-dihydroxy-3H-spiro[isobenzofuran-1,9′-xanthen]-3-one (cas: 76-54-0COA of Formula: C20H10Cl2O5).
2′,7′-Dichloro-3′,6′-dihydroxy-3H-spiro[isobenzofuran-1,9′-xanthen]-3-one (cas: 76-54-0) belongs to benzofurans derivatives. Benzofurans are compounds with a planar structure having 10 pi electrons that include the lone pair on oxygen atom, which makes it more susceptible to electrophilic attack. Introduction of benzofurans in organic synthesis, particularly drug synthesis, involves generally the use of their metalated species as nucleophiles in addition reactions or in metal-catalysed cross-coupling reactions.COA of Formula: C20H10Cl2O5
Referemce:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem