Cholinergic system and exploratory behavior are changed after weekly-binge ethanol exposure in zebrafish was written by Bernardo, Henrique Teza;Agostini, Jotele Fontana;Toe, Helena Cristina Zuehl Dal;Vieira, Karine Medeiros;Baldin, Samira Leila;Schuck, Patricia Fernanda;Uribe-Cruz, Carolina;Longo, Larisse;da Silveira, Themis Reverbel;Rosemberg, Denis Broock;Rico, Eduardo Pacheco. And the article was included in Pharmacology, Biochemistry and Behavior in 2019.Synthetic Route of C20H10Cl2O5 This article mentions the following:
Binge drinking is characterized by excessive alc. consumption in a short period of time and is associated with a poor quality of life. Zebrafish are commonly used to investigate neurochem., behavioral, and genetic parameters associated with ethanol (EtOH) exposure. However, few studies have used zebrafish as a model to investigate binge EtOH exposure. In order to elucidate the potential neurobehavioral impairments evoked by binge EtOH exposure in zebrafish, animals were immersed in 1.4% EtOH for 30 min three consecutive times with intervals of one week. Neurobehavioral parameters were analyzed immediately following the third exposure, as well as 2 and 9 days later. Brain choline acetyltransferase (ChAT) and acetylcholinesterase (AChE) activities were reduced 9 days after the treatment. Thiobarbituric acid-reactive species and dichlorodihydrofluorescein levels were increased immediately after the treatment, but both returned to normal levels 2 days after the treatment. Catalase and glutathione reductase were impaired 2 and 9 days after the treatment. No alteration was observed in superoxide dismutase and glutathione peroxidase activities. EtOH treatment did not alter brain expression of inflammatory genes such as il-1β, il-10, and tnf-α . Zebrafish displayed anxiolytic-like behavior immediately after the last exposure, though there was no behavioral alteration observed 9 days after the treatment. Therefore, binge EtOH exposure in zebrafish leads to long lasting brain cholinergic alteration, probably related to oxidative stress immediately after the exposure, which is independent of classical inflammatory markers. In the experiment, the researchers used many compounds, for example, 2′,7′-Dichloro-3′,6′-dihydroxy-3H-spiro[isobenzofuran-1,9′-xanthen]-3-one (cas: 76-54-0Synthetic Route of C20H10Cl2O5).
2′,7′-Dichloro-3′,6′-dihydroxy-3H-spiro[isobenzofuran-1,9′-xanthen]-3-one (cas: 76-54-0) belongs to benzofurans derivatives. Benzofuran is a core structural unit found in many naturally occurring compounds with multidirectional biological activities. In nature, benzofurans have occupied an important role among the plant phenols & several pharmacologically active compounds.Synthetic Route of C20H10Cl2O5
Referemce:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem