Production and initial application of carbofuran polyclonal antibody was written by Ben, Ya-li;Zhu, De-rui;Hu, Qin-qin;Li, Na;Xiong, Li;Liu, De-li. And the article was included in Huanjing Kexue Yanjiu in 2008.Category: benzofurans This article mentions the following:
A hapten 6-[[(2, 3-dihydro-2, 2-dimetyl-7-benzofuranyloxy) carbonyl] amino] hexanoic acid (BFNH) completely preserving the carbamate group of carbofuran was synthesized with 2, 3-dihydro-2, 2-dimethyl-7-benzofuranol and aminocaproic acid, which was activated with 4-nitrophenyl chloroformate. The BFNH hapten was attached to bovine serum albumin (BSA) by utilizing the modified active ester method to synthesize antigen BFNH-BSA. The BFNH-BSA was used as immunogen, with which an anti-carbofuran polyclonal antibody (pAb) was successfully obtained by immunization of rabbits. The coupling ratios of BFNH-BSA and BFNH-OVA were 18:1 and 5:1, resp. The highest titer of the pAb was 5.12 x 106 by purifying rabbit II with the caprylic acid method. The linear concentrations ranged from 10-7 mg/mL to 0.01 mg/mL and the detection limit was 10-7mg/mL for the determination of carbofuran by a competitive indirect ELISA procedure. The cross-reactivities of five oxamic acid esters were lower than 10 %. The pAb was applied initially to detect carbofuran reside in fruit and vegetables. In the experiment, the researchers used many compounds, for example, 2,2-Dimethyl-2,3-dihydrobenzofuran-7-ol (cas: 1563-38-8Category: benzofurans).
2,2-Dimethyl-2,3-dihydrobenzofuran-7-ol (cas: 1563-38-8) belongs to benzofurans derivatives.Benzofuran is one of the most significant oxygen-containing heterocycles consisting of fused benzene and furan ring, which are widely presented in various naturally occurring and synthetically active compounds. They are also prone to polymerisation in the presence of concentrated mineral acids and Lewis acids.Category: benzofurans
Referemce:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem