Alternative sample treatments for the determination of sulfonamides in milk by HPLC with fluorescence detection was written by Arroyo-Manzanares, Natalia;Gamiz-Gracia, Laura;Garcia-Campana, Ana M.. And the article was included in Food Chemistry in 2014.Synthetic Route of C17H10O4 This article mentions the following:
Sample treatments by dispersive liquid-liquid microextraction (DLLME) and QuEChERS for the determination of 9 sulfonamides in milk were compared. Both sample preparation methods represent useful alternatives to conventional procedures based mainly on solid-phase extraction, in terms of simplicity, decreased use of organic solvents, sample throughput, and effectiveness in extract clean-up. The sample preparation methods were compared for efficiency, trueness, sensitivity and precision using HPLC with fluorescamine derivatization and fluorescence detection. The clean extracts were obtained with analyte recoveries 90.8-104.7 and 83.6-104.8% for DLLME and QuEChERS, resp. Matrix-matched calibration curves were established for both methods using milk samples spiked at 4 concentration levels. LODs (3xS/N) <1.21 and 2.73 μg/L for DLLME and QuEChERS, resp., were obtained in all cases. The precision, in terms of repeatability and intermediate precision, was <10% in all cases. In the experiment, the researchers used many compounds, for example, 4-Phenyl-3H,3’H-spiro[furan-2,1′-isobenzofuran]-3,3′-dione (cas: 38183-12-9Synthetic Route of C17H10O4).
4-Phenyl-3H,3’H-spiro[furan-2,1′-isobenzofuran]-3,3′-dione (cas: 38183-12-9) belongs to benzofurans derivatives. Benzofuran is a core structural unit found in many naturally occurring compounds with multidirectional biological activities. Benzofurans are stable towards alkali and readily polymerize on treatment with sulfuric acid, due to which they are useful for the preparation of low cost chemically relatively inert resins.Synthetic Route of C17H10O4
Referemce:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem