Simultaneous characterization of two types of major active components in Kushen by high performance liquid chromatography coupled to multi-stage mass spectrometry was written by Zhao, Qinqin;Zhang, Yufeng;Fan, Xiaohui. And the article was included in Zhongguo Zhongyao Zazhi in 2011.Reference of 6807-83-6 This article mentions the following:
In this study, a high performance liquid chromatog. coupled with multi-stage mass spectrometry method (HPLC-MS) was developed for simultaneous characterization of alkaloids and flavonoids, the main active components, in Kushen with diverse phys. and chem. properties. Forty-two major constituents, including sixteen Kushen alkaloids and twenty-six Kushen flavonoids were tentatively identified. Addnl., useful and characteristic fragmentation pathways of two types of Kushen alkaloids, namely cytisine-type and sparteine-type, in pos. ions mode were proposed and summarized, which would lay a foundation for the rapid identification of the active ingredients in traditional Chinese medicine Kushen. In the experiment, the researchers used many compounds, for example, (2S,3R,4S,5S,6R)-2-(((6aR,12aR)-6a,12a-Dihydro-6H-[1,3]dioxolo[4′,5′:5,6]benzofuro[3,2-c]chromen-3-yl)oxy)-6-(hydroxymethyl)tetrahydro-2H-pyran-3,4,5-triol (cas: 6807-83-6Reference of 6807-83-6).
(2S,3R,4S,5S,6R)-2-(((6aR,12aR)-6a,12a-Dihydro-6H-[1,3]dioxolo[4′,5′:5,6]benzofuro[3,2-c]chromen-3-yl)oxy)-6-(hydroxymethyl)tetrahydro-2H-pyran-3,4,5-triol (cas: 6807-83-6) belongs to benzofurans derivatives. Benzofuran is a core structural unit found in many naturally occurring compounds with multidirectional biological activities. As benzofurans are prone to undergo ring opening of the heterocycle, examples of reduction of this type of aromatics by using dissolving metals are rather scarce.Reference of 6807-83-6
Referemce:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem