Development of luminol-fluorescamine-PVP chemiluminescence system and its application to sensitive tyrosinase determination was written by Wang, Chao;Chen, Yequan;Snizhko, Dmytro;Du, Fangxin;Ma, Xiangui;Lou, Baohua;Li, Jianping;Xu, Guobao. And the article was included in Talanta in 2020.COA of Formula: C17H10O4 This article mentions the following:
Fluorescamine is a popular fluorescent probe. We report for the first time that luminol chemiluminescence (CL) can be enhanced by fluorescamine in the presence of PVP. The CL intensity of luminol-fluorescamine-PVP is about 26 times stronger than that of luminol. Both the removal of oxygen and the addition of superoxide dismutase (SOD) decrease CL intensity, thiourea and NaN3 have little effect on CL intensities, indicating that Oâ?2 is critical for CL. Interestingly, o-quinone generated from phenol by tyrosinase obviously inhibited the CL intensity. Inspired by such quenching effect on the luminol-fluorescamine-PVP CL system, a sensitive CL sensing for the determination of tyrosinase activity was developed. The method can detect tyrosinase in the range of 0.07-1.5μg mL-1 (0.19-4.02 U mL-1) with the detection limit of 0.035μg mL-1 (0.094 U mL-1). Moreover, this method exhibits satisfied recoveries for the spiked human serum samples. In the experiment, the researchers used many compounds, for example, 4-Phenyl-3H,3’H-spiro[furan-2,1′-isobenzofuran]-3,3′-dione (cas: 38183-12-9COA of Formula: C17H10O4).
4-Phenyl-3H,3’H-spiro[furan-2,1′-isobenzofuran]-3,3′-dione (cas: 38183-12-9) belongs to benzofurans derivatives. Many natural or synthetic compounds containing benzofuran skeletons have been found to possess remarkable activity as agrochemicals and pharmaceuticals. Introduction of benzofurans in organic synthesis, particularly drug synthesis, involves generally the use of their metalated species as nucleophiles in addition reactions or in metal-catalysed cross-coupling reactions.COA of Formula: C17H10O4
Referemce:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem