Direct detection of nitrotyrosine-containing proteins using an aniline-based oxidative coupling strategy was written by Sangsuwan, Rapeepat;Obermeyer, Allie C.;Tachachartvanich, Phum;Palaniappan, Krishnan K.;Francis, Matthew B.. And the article was included in Chemical Communications (Cambridge, United Kingdom) in 2016.Application of 3326-34-9 This article mentions the following:
A convenient two-step method is described for the detection of nitrotyrosine-containing proteins. First, nitrotyrosines are reduced to aminophenols using sodium dithionite. Following this, an oxidative coupling reaction is used to attach anilines bearing fluorescence reporters or affinity probes. Features of this approach include fast reaction times, pmol-level sensitivity, and excellent chemoselectivity. In the experiment, the researchers used many compounds, for example, 5-Amino-3′,6′-dihydroxy-3H-spiro[isobenzofuran-1,9′-xanthen]-3-one (cas: 3326-34-9Application of 3326-34-9).
5-Amino-3′,6′-dihydroxy-3H-spiro[isobenzofuran-1,9′-xanthen]-3-one (cas: 3326-34-9) belongs to benzofurans derivatives. Many natural or synthetic compounds containing benzofuran skeletons have been found to possess remarkable activity as agrochemicals and pharmaceuticals. Introduction of benzofurans in organic synthesis, particularly drug synthesis, involves generally the use of their metalated species as nucleophiles in addition reactions or in metal-catalysed cross-coupling reactions.Application of 3326-34-9
Referemce:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem