《Construction of FRET-Based Off-On Fluorescent Nanoprobes for Sensitive Detection of Intracellular Singlet Oxygen》 was written by Ping, Jian-tao; Qin, Jing-lei; Zhou, Chao; Guo, Lan-ying; Liu, Bei; Chen, Zhi-hua; Geng, Zhao-xin; Peng, Hong-shang. Computed Properties of C20H14O And the article was included in ChemNanoMat in 2020. The article conveys some information:
Singlet oxygen (1O2) detection remains an active area of research in the field of biol. and biomedical science. In this work, we reported a novel Off-On type FRET-based fluorescent 1O2 nanoprobe comprising the energy-donating conjugated polymer, poly(9,9-dioctylfluorenyl-2,7-diyl) (PFO), and the energy-accepting 1O2 indicator, 3-diphenylisobenzofuran (DPBF). Prior to the reaction with 1O2, the nanoprobe was in Off-state because fluorescence of PFO was completely quenched by loaded DPBF; in the presence of 1O2, it switched to On-state due to the depletion of DPBF. The as-prepared 1O2 nanoprobe had a particle size of ~59 nm, good biocompatibility, high specificity and sensitivity with a limit of detection of 353 nM. Importantly, the fluorescent nanoprobes were highly sensitive to 1O2 under two-photon excitation. Using a multiphoton confocal microscope, generation of intracellular 1O2 during photodynamic therapy was sensitively detected by recording fluorescence intensity of the nanoprobes. In the experiment, the researchers used many compounds, for example, 1,3-Diphenylisobenzofuran(cas: 5471-63-6Computed Properties of C20H14O)
1,3-Diphenylisobenzofuran(cas: 5471-63-6) can be used as a fluorescent probe for detection of superoxide anion radical (O2−) inside the membrane lipid layer by DPBF fluorescence quenching method. 1,3-Diphenylisobenzofuran(DPBF) can be used as quencher during the photoinactivation of TA-3 mouse mammary carcinoma cells containing hematoporphyrin.Computed Properties of C20H14O
Referemce:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem