Machine Learning in Chemistry about 90866-33-4

Although many compounds look similar to this compound(90866-33-4)Reference of (R)-Ethyl 4-chloro-3-hydroxybutanoate, numerous studies have shown that this compound(SMILES:O=C(OCC)C[C@@H](O)CCl), has unique advantages. If you want to know more about similar compounds, you can read my other articles.

Reference of (R)-Ethyl 4-chloro-3-hydroxybutanoate. The protonation of heteroatoms in aromatic heterocycles can be divided into two categories: lone pairs of electrons are in the aromatic ring conjugated system; and lone pairs of electrons do not participate. Compound: (R)-Ethyl 4-chloro-3-hydroxybutanoate, is researched, Molecular C6H11ClO3, CAS is 90866-33-4, about Development of Saccharomyces cerevisiae reductase YOL151W mutants suitable for chiral alcohol synthesis using an NADH cofactor regeneration system. Author is Yoon, Shin Ah; Jung, Jihye; Park, Seongsoon; Kim, Hyung Kwoun.

The aldo-keto reductases catalyze reduction reactions using various aliphatic and aromatic aldehydes/ketones. Most reductases require NADPH exclusively as their cofactors. However, NADPH is much more expensive and unstable than NADH. In this study, we attempted to change the five amino acid residues that interact with the 2′-phosphate group of the adenosine ribose of NADPH. These residues were selected based on a docking model of the YOL151W reductase and were substituted with other amino acids to develop NADH-utilizing enzymes. Ten mutants were constructed by site-directed mutagenesis and expressed in Escherichia coli. Among them, four mutants showed higher reductase activities than wild-type when using the NADH cofactor. Anal. of the kinetic parameters for the wild type and mutants indicated that the kcat/Km value of the Asn9Glu mutant toward NADH increased 3-fold. A docking model was used to show that the carboxyl group of Glu 9 of the mutant formed an addnl. hydrogen bond with the 2′-hydroxyl group of adenosine ribose. The Asn9Glu mutant was able to produce (R)-ethyl-4-chloro-3-hydroxyl butenoate rapidly when using the NADH regeneration system.

Although many compounds look similar to this compound(90866-33-4)Reference of (R)-Ethyl 4-chloro-3-hydroxybutanoate, numerous studies have shown that this compound(SMILES:O=C(OCC)C[C@@H](O)CCl), has unique advantages. If you want to know more about similar compounds, you can read my other articles.

Reference:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem