Establishment of HPLC fingerprint of Sophora tonkinensis var. polyphylla from Guangxi and comparison of concordance with HPLC fingerprint of S. tonkinensis was written by Liu, Ji-cheng;Lu, Sen-hua;Xie, Wei;Lu, Wen-jie. And the article was included in Zhongguo Shiyan Fangjixue Zazhi in 2014.Formula: C22H22O10 This article mentions the following:
Objective: To establish the HLPC fingerprint method of Sophora tonkinensis var. polyphylla, to provide the reference for scientific evaluation and quality control of S. tonkinensis var. polyphylla herb, and to provide the reference data for the homol. study of Sophora tonkinensis var. polyphylla and S. tonkinensis through comparing the fingerprints. Method: An HPLC method was used. An Agilent Eclipse XDB-C18 (4.6 mm × 250 mm, 5 μm) column was adopted, and the mobile phase consisted of acetonitrile and 0.05 mol·L-1 potassium dihydrogen phosphate solution (adjusted with triethylamine pH 6.4) with a gradient elution. The flow rate was 1.0 mL·min-1, the column temperature was 30°C, and the detection wavelength was 215 nm. A ‘Chromatog. Fingerprint Evaluation System 2004A Edition’ software was used for data processing. Result: The establishment fingerprint of S. tonkinensis var. polyphylla had 14 common peaks and 7 peaks of them were identified using the reference retention time and UV-DAD detector extracted spectra dual qual. indicators. Most of the compare similarities of fingerprints of each batch herb were above 0.90, in line with the requirements of fingerprint technol. research. Compared with the fingerprint of S. tonkinensis var. polyphylla and S. tonkinensis, the similarity was above 0.90. Conclusion: The precision, stability and reproducibility of the established method were good. The method could be effectively used for the quality control of S. tonkinensis var. polyphylla herb and provide a scientific basis for the development and use of the herb. It could be inferred that S. tonkinensis var. polyphylla and S. tonkinensis had similar ingredients and close relationships from the comparison study of their fingerprints. In the experiment, the researchers used many compounds, for example, (2S,3R,4S,5S,6R)-2-(((6aR,12aR)-6a,12a-Dihydro-6H-[1,3]dioxolo[4′,5′:5,6]benzofuro[3,2-c]chromen-3-yl)oxy)-6-(hydroxymethyl)tetrahydro-2H-pyran-3,4,5-triol (cas: 6807-83-6Formula: C22H22O10).
(2S,3R,4S,5S,6R)-2-(((6aR,12aR)-6a,12a-Dihydro-6H-[1,3]dioxolo[4′,5′:5,6]benzofuro[3,2-c]chromen-3-yl)oxy)-6-(hydroxymethyl)tetrahydro-2H-pyran-3,4,5-triol (cas: 6807-83-6) belongs to benzofurans derivatives. Benzofuran derivatives are one of the most important oxygen-containing heterocycles. Introduction of benzofurans in organic synthesis, particularly drug synthesis, involves generally the use of their metalated species as nucleophiles in addition reactions or in metal-catalysed cross-coupling reactions.Formula: C22H22O10
Referemce:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem