Development of transgenic hairy roots and augmentation of secondary metabolites by precursor feeding in Sphagneticola calendulacea (L.) Pruski was written by Kundu, Suprabuddha;Salma, Umme;Ali, Nasim Md.;Hazra, Alok Kumar;Mandal, Nirmal. And the article was included in Industrial Crops and Products in 2018.Reference of 524-12-9 The following contents are mentioned in the article:
Sphagneticola calendulacea (L.) Pruski [synonym Wedelia chinensis (Osbeck) Merrill] is an important medicinal plant that possesses a variety of bioactive secondary metabolites. A stable transgenic hairy root culture has been developed using engineered Agrobacterium rhizogenes strain LBA1334 harboring pCAM:2 × 35S:gusA binary vector, to achieve high yield of secondary metabolites. Putative transgenic hairy roots appeared with highest transformation frequency (66.67%) when leaf explants were immersed in A. rhizogenes suspension for 10 min and co-cultivated for three days. Incorporation of foreign genes was confirmed by initial GUS staining assay followed by polymerase chain reaction anal. of rolA, rolB, rolC and gusA genes. Time course study revealed that the transgenic hairy roots grew rapidly in 1/2 Murashige and Skoog liquid medium with highest biomass production of 0.61 g per 50 mL dry weight after 28 days of culture. In the precursor feeding experiment, the biomass of hairy roots was further increased to 0.698 g per 50 mL dry weight when 0.5 mM phenylalanine was added to the 10 days old growing culture. High Performance Thin Layer Chromatog. showed that the transgenic hairy roots accumulated enhanced level of wedelolactone (422.01μg g-1 dry weight), which was 1.43 and 1.37 times higher than those of in vivo (294.44μg g-1 dry weight) and in vitro-grown (308.28μg g-1 dry weight) plantlets, resp. Precursor feeding with phenylalanine further augmented the accumulation of wedelolactone to 440.33μg g-1 dry weight in the transgenic hairy roots. In addition, the content of several important phenolic and flavonoid compounds were increased in the transgenic hairy roots as analyzed by High Performance Liquid Chromatog. The protocol offers a scope to introduce foreign genes and further regulate the production of secondary metabolites. This study involved multiple reactions and reactants, such as 1,8,9-Trihydroxy-3-methoxy-6H-benzofuro[3,2-c]chromen-6-one (cas: 524-12-9Reference of 524-12-9).
1,8,9-Trihydroxy-3-methoxy-6H-benzofuro[3,2-c]chromen-6-one (cas: 524-12-9) belongs to benzofurans derivatives. Benzofuran is a core structural unit found in many naturally occurring compounds with multidirectional biological activities. As benzofurans are prone to undergo ring opening of the heterocycle, examples of reduction of this type of aromatics by using dissolving metals are rather scarce.Reference of 524-12-9
Referemce:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem