Altered expression and function of hepatic transporters in a rodent model of polycystic kidney disease was written by Bezencon, Jacqueline;Beaudoin, James J.;Ito, Katsuaki;Fu, Dong;Roth, Sharin E.;Brock, William J.;Brouwer, Kim L. R.. And the article was included in Drug Metabolism & Disposition in 2019.Formula: C20H10Cl2O5 This article mentions the following:
Autosomal dominant polycystic kidney disease (ADPKD) is a common form of inherited polycystic kidney disease (PKD) and is a leading cause of kidney failure. Patients with ADPKD develop fluid-filled cysts in their kidneys, and often form cysts in their liver and other organs. However, the impact of PKD on hepatic transporters has not been characterized. Therefore, this preclin. study was designed to investigate hepatic transporter expression and function in PCK compared to wild-type (WT) Sprague Dawley rats. Transporter gene expression was measured by qPCR, and protein expression was quantified by western blot and LC-MS/MS-based proteomic anal. in rat livers. Transporter function was assessed in isolated perfused livers (IPLs), and biliary and hepatic total glutathione content were measured. Protein expression of Mrp2 and Oatp1a4 was decreased 3-fold and 2.9-fold, resp., in PCK rat livers based on western blot anal. Proteomic anal. confirmed a decrease in Mrp2 and a decrease in Oatp1a1 expression (PCK/WT ratios of 0.368[plusmn]0.098 and 0.563[plusmn]0.038, resp.; mean[plusmn]SD). The biliary excretion of 5(6)-carboxy-2′,7′-dichlorofluorescein (CDF), a substrate of Oatp1a1, Mrp2, and Mrp3, was decreased 28-fold in PCK compared to WT rat IPLs. Total glutathione was significantly reduced in the bile of PCK rats. Differences in hepatic transporter expression and function may contribute to altered disposition of Mrp2 and Oatp substrates in PKD. In the experiment, the researchers used many compounds, for example, 2′,7′-Dichloro-3′,6′-dihydroxy-3H-spiro[isobenzofuran-1,9′-xanthen]-3-one (cas: 76-54-0Formula: C20H10Cl2O5).
2′,7′-Dichloro-3′,6′-dihydroxy-3H-spiro[isobenzofuran-1,9′-xanthen]-3-one (cas: 76-54-0) belongs to benzofurans derivatives.Benzofuran is one of the most significant oxygen-containing heterocycles consisting of fused benzene and furan ring, which are widely presented in various naturally occurring and synthetically active compounds. As benzofurans are prone to undergo ring opening of the heterocycle, examples of reduction of this type of aromatics by using dissolving metals are rather scarce.Formula: C20H10Cl2O5
Referemce:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem