Metabolomics approach based on ultra-high-performance liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry to identify the chemical constituents of the Traditional Chinese Er-Zhi-Pill was written by Zhong, Xunlong;Zhong, Yanmei;Yan, Kangqi;Xiao, Xuerong;Duan, Lian;Wang, Ruolun;Wang, Laiyou. And the article was included in Journal of Separation Science in 2017.Category: benzofurans The following contents are mentioned in the article:
Er-Zhi-Pill, which consists of Ligustri lucidi fructus and Ecliptae prostratae herba, is a classical traditional Chinese medicinal formulation widely used as a liver-nourishing and kidney-enriching tonic. To identify the bioactive ingredients of Er-Zhi-Pill and characterize the variation of chem. constituents between co-decoction and mix of individually decocted L. lucidi fructus and E. prostratae herba, a novel metabolomics approach based on ultra high performance liquid chromatog. coupled with quadrupole time-of-flight mass spectrometry in both pos. and neg. ion modes, was established to comprehensively analyze chem. constituents and probe distinguishable chem. markers. In total, 68 constituents were unambiguously or tentatively identified through alignment of accurate mol. weights within an error margin of 5 ppm, elemental composition and fragmentation characteristics, including eight constituents, which were confirmed by comparing to reference standards Furthermore, principal component anal. and partial least squares discriminant anal. using Simca-p+ 12.0 software were applied to investigate chem. differences between formulations obtained by co-decoction and a mixture of individual decoctions. Global chem. differences were found in samples of two different decoction methods, and 16 components, including salidroside, specneuzhenide and wedelolactone, contributed most to the observed differences. This study provides a basic chem. profile for the quality control and further mechanism research of Er-Zhi-Pill. This study involved multiple reactions and reactants, such as 1,8,9-Trihydroxy-3-methoxy-6H-benzofuro[3,2-c]chromen-6-one (cas: 524-12-9Category: benzofurans).
1,8,9-Trihydroxy-3-methoxy-6H-benzofuro[3,2-c]chromen-6-one (cas: 524-12-9) belongs to benzofurans derivatives. Many natural or synthetic compounds containing benzofuran skeletons have been found to possess remarkable activity as agrochemicals and pharmaceuticals. They are also prone to polymerisation in the presence of concentrated mineral acids and Lewis acids.Category: benzofurans
Referemce:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem